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KMID : 0380620130450020241
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 2 p.241 ~ p.247
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Intra-laboratory Validation of an HPLC Post-column Oxidation Method for the Analysis of PSP Toxins in Oysters and Mussels
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Song Ki-Cheol
Lee Ka-Jung
Yu Hong-Sik
Mok Jong-Soo
Kim Ji-Hoe
Lim Keun-Sik
Lee Mi-Ae
Kim Mee-Hye
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Abstract
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AOAC Mouse Bioassay Analysis (MBA) has been the gold standard for the analysis of paralytic shellfish poisoning toxin (PSP toxin) for more than 50 years. However, this method has inaccurate limit of quantification and cannot be used to determine toxic profiles. An HPLC method (PCOX) was optimized for Korean shellfish to establish an alternative or supplementary method for PSP analysis and was intended to be used for the official monitoring and regulation of food. The recovery rate of the PCOX method was 83.5-112.1% and the limit of quantification for total toxin was about 8.6 μg/100 g. A long-term comparison study showed a good correlation of the PCOX results with the AOAC MBA results: the correlation factors were 0.9534 and 0.9109 for oyster and mussel matrices, respectively. The PCOX method may be used as an alternative or supplementary method for AOAC MBA to monitor the occurrence of PSP and to analyze PSP toxin profile in oysters and mussels.
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KEYWORD
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paralytic shellfish poisoning toxin, mouse bioassay analysis, post-column oxidation, HPLC
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